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Molecular characterization of resistance responses of Coffea canephora "Clone 14" upon infection with Meloidogyne paranaensis

Lima E.A., Carneiro F.A., Costa T.S., Rego E.C.S., Jorge A., Furlanetto C., Marraccini P., Carneiro R.M.D.G., Andrade A.C.. 2014. Journal of Nematology, 46 (2) : p. 194-194. International Congress of Nematology. 6, Le Cap (Afrique du Sud).

Coffee is one of the major commodities in the world and an important source of income for producing countries. However, biotic and abiotic stresses are great limiting factors to coffee yield. In Brazil, root-knot nematodes cause considerable yield reduction and the use of resistant plants is themost promising method to control Meloidogyne spp. The aim of this work was to characterize the molecular mechanism underlining the previously identified resistance to M. paranaensis in C. canephora 'Clone 14' by means of RNAseq experiments. Differential expression using RNA extracted from roots of plants from clones 14 and clone 22 of C. canephora, previously identified as resistant and susceptible to M. paranaensis, respectively, were grown in sand and inoculated. Root samples were collected at different time points post inoculation as well as roots from an uninfected plant. The RNAwas treated with DNAse and subsequently, a portion of the sample was lyophilized for RNAseq experiments and another portion kept for validation by qPCR experiments. Results of the identified candidate genes with differential expression among resistant {Clone 14) and susceptible (Clone 22) genotypes will be presented and discussed. (Texte intégral)

Mots-clés : coffea canephora; brésil

Thématique : Ravageurs des plantes; Génétique et amélioration des plantes; Physiologie et biochimie végétales

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