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Metagenomics approaches based on virion-associated nucleic acids (VANA): an innovative tool for assessing without A priori viral diversity of plants

Filloux D., Dallot S., Delaunay A., Galzi S., Jacquot E., Roumagnac P.. 2015. In : Lacomme Christophe (ed.). Plant pathology: techniques and protocols. New York : Springer [Etats-Unis], p. 249-257. (Methods in Molecular Biology, 1302).

DOI: 10.1007/978-1-4939-2620-6_18

This chapter describes an efficient approach that combines quality and yield extraction of viral nucleic acids from plants containing high levels of secondary metabolites and a sequence-independent amplification procedure for both the inventory of known plant viruses and the discovery of unknown ones. This approach turns out to be a useful tool for assessing the virome (the genome of all the viruses that inhabit a particular organism) of plants of interest. We here show that this approach enables the identification of a novel Potyvirus member within a single plant already known to be infected by two other Potyvirus species.

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