Publications des agents du Cirad

Cirad

Protective effect of tropical highland blackberry juice (Rubus adenotrichos Schltdl.) against UVB-mediated damage in human epidermal keratinocytes and in a reconstituted skin equivalent model

Calvo-Castro L., Syed D.N., Chamcheu J.C., Vilela F.M.P., Pérez A.M., Vaillant F., Rojas M., Mukhtar H.. 2013. Photochemistry and Photobiology, 89 (5) : p. 1199-1207.

Solar ultraviolet (UV) radiation, particularly its UVB (280¿320 nm) spectrum, is the primary environmental stimulus leading to skin carcinogenesis. Several botanical species with antioxidant properties have shown photochemopreventive effects against UVB damage. Costa Rica's tropical highland blackberry (Rubus adenotrichos) contains important levels of phenolic compounds, mainly ellagitannins and anthocyanins, with strong antioxidant properties. In this study, we examined the photochemopreventive effect of R. adenotrichos blackberry juice (BBJ) on UVB-mediated responses in human epidermal keratinocytes and in a three-dimensional (3D) reconstituted normal human skin equivalent (SE). Pretreatment (2 h) and posttreatment (24 h) of normal human epidermal keratinocytes (NHEKs) with BBJ reduced UVB (25 mJ cm?2)-mediated (1) cyclobutane pyrimidine dimers (CPDs) and (2) 8-oxo-7,8-dihydro-2?-deoxyguanosine (8-oxodG) formation. Furthermore, treatment of NHEKs with BBJ increased UVB-mediated (1) poly(ADP-ribose) polymerase cleavage and (2) activation of caspases 3, 8 and 9. Thus, BBJ seems to alleviate UVB-induced effects by reducing DNA damage and increasing apoptosis of damaged cells. To establish the in vivo significance of these findings to human skin, immunohistochemistry studies were performed in a 3D SE model, where BBJ was also found to decrease CPDs formation. These data suggest that BBJ may be developed as an agent to ameliorate UV-induced skin damage. (Résumé d'auteur)

Mots-clés : souris; médicament; dermatologie; peau; composé phénolique; antioxydant; rayonnement ultraviolet; jus de fruits; rubus; costa rica; rubus adenotrichos

Thématique : Traitement des produits agricoles non alimentaires; Autres thèmes

Documents associés

Article de revue

Agents Cirad, auteurs de cette publication :