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Binding sites of mirnas with transcription factors' genes of Camelus ferus

Alybaeva A., Berillo O., Niyazova R., Faye B., Ivashchenko A.. 2015. Veterinariâ, 42 (2) : p. 340-342. 4th Conference of the International Society of Camelid Research and Development/ISOCARD 2015. 4, 2015-06-08/2015-06-12, Almaty (Kazakhstan).

We searched binding sites of miRNAs in mRNAs of 157 transcription factors' genes of wild camel (Camelus ferus). The mRNAs of 96 genes of zincfinger transcription factors' family have 16, 210 and 34 binding sites in the 5'UTRs, CDSs and 3'UTRs, respectively. The mRNA of GLI2 gene has binding sites for eight miRNAs. The mRNAs of GLIS1 and ZNF236 genes contain seven binding sites. In the 3'UTR mRNA of ZFP91 gene were revealed 13 miR-574-5p binding sites arranged located through two nucleotides. The ?G/?Gm value is equal to 93%. miR-1322 has one binding site in GLI1, HINFP, HIVEP1, MTF1, SALL4, SP1, ZNF335 and ZNF451 genes, two sites in ZNF142, three sites in EGR1 gene. mRNA of VEZF1 gene has eight miR-1322 binding sites arranged located through three nucleotides. miRNAs with the length of 25 and 26 nucleotides have the highest binding energy. The ?G value varied from -114,6 kJ/mole to -138,0 kJ/mole. Some miRNAs with a length of 23 and 24 nucleotides also have a high value ?G varied from -112,5 kJ/mole to -129,5 kJ/mole. The results show a strong interaction between the expression of genes of transcription factors and miRNAs. (Résumé d'auteur)

Mots-clés : genre humain; expression des gènes; facteur de transcription; arn; transcription génique; animal sauvage; camelus; camelus ferus

Thématique : Génétique et amélioration des animaux

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