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Whole blood transcriptome analysis of Mycoplasma mycoides subsp. mycoides-infected cattle confirms immunosuppression but does not reflect local inflammation

Rodrigues V., Holzmuller P., Puech C., Wesonga H., Thiaucourt F., Manso-Silvan L.. 2015. PloS One, 10 (10) : 18 p..

Contagious bovine pleuropneumonia (CBPP), caused by Mycoplasma mycoides subsp. mycoides (Mmm) , is a severe respiratory disease of cattle responsible for major economic losses in sub-Saharan Africa. Disease control relies mainly on the use of empirically attenu- ated vaccines that provide limited protection. Thus, understanding the virulence mechanisms used by Mmm as well as the role of the host immune system in disease development, persis- tence, and control is a prerequisite for the development of new, rationally designed control strategies. The aim of this study was to assess the use of whole blood transcriptome analysis to study cattle- Mmm interactions, starting by the characterization of the bovine response to Mmm infection during the acute form of the disease. For that purpose, we compared the tran- scriptome profile of whole blood from six cattle, before challenge by contact with Mmm - infected animals and at the appearance of first clinical signs, using a bovine microarray. Func- tional analysis revealed that 680 annotated genes were differentially expressed, with an over- whelming majority of down-regulated genes characterizing an immunosuppression. The main bio-functions affected were ¿ organismal survival ¿ , ¿ cellular development , morphology and functions ¿ and ¿ cell-to cell signaling and interactions ¿ . These affected functions were consis- tent with the results of previous in vitro immunological studies. However, microarray and qPCR validation results did not highlight pro-inflammatory molecules (such as TNF ? , TLR2, IL-12B and IL-6), whereas inflammation is one of the most characteristic traits of acute CBPP. This global gene expression pattern may be considered as the result, in blood, of the local pulmo- nary response and the systemic events occurring during acute CBPP. Nevertheless, to under- stand the immune events occurring during disease, detailed analyses on the different immune cell subpopulations, either in vivo , at the local site, or in vitro , will be required. Whole blood tran- scriptome analysis remains an interesting approach for the identification of bio-signatures cor- relating to recovery and protection, which should facilitate the evaluation and validation of novel vaccine formulations. (Résumé d'auteur)

Thématique : Maladies des animaux

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