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Building the sugarcane genome for biotechnology and identifying evolutionary trends

de Setta N., Monteiro-Vitorello C., Metcalfe C.J., Cruz G.M.Q., Del Bem L.E., Vicentini R., Tebaldi Silveira Nogueira F., Alvares Campos R., Lima Nunes S., Gasperazzo Turrini P.C., Prata Vieira A., Ochoa Cruz E.A., Silveira Corrêa T.C., Takeshi Hotta C., de Mello Varani A., Vautrin S., Silva da Trindade A., de Mendonça Vilela M., Gimiliani Lembke C., Mieko Sato P., Fandino de Andrade R., Yutaka Nishiyama M., Cardoso-Silva C.B., Castanho Scortecci K., Garcia A.A.F., Sampaio Carneiro M., Kim C., Paterson A.H., Berges H., D'Hont A., Pereira de Souza A., Mendes Souza G., Vincentz M., Kitajima J.P., Van Sluys M.A.. 2014. BMC Genomics, 15 (540) : 18 p..

Background Sugarcane is the source of sugar in all tropical and subtropical countries and is becoming increasingly important for bio-based fuels. However, its large (10 Gb), polyploid, complex genome has hindered genome based breeding efforts. Here we release the largest and most diverse set of sugarcane genome sequences to date, as part of an on-going initiative to provide a sugarcane genomic information resource, with the ultimate goal of producing a gold standard genome. Results Three hundred and seventeen chiefly euchromatic BACs were sequenced. A reference set of one thousand four hundred manually-annotated protein-coding genes was generated. A small RNA collection and a RNA-seq library were used to explore expression patterns and the sRNA landscape. In the sucrose and starch metabolism pathway, 16 non-redundant enzyme-encoding genes were identified. One of the sucrose pathway genes, sucrose-6-phosphate phosphohydrolase, is duplicated in sugarcane and sorghum, but not in rice and maize. A diversity analysis of the s6pp duplication region revealed haplotype-structured sequence composition. Examination of hom(e)ologous loci indicate both sequence structural and sRNA landscape variation. A synteny analysis shows that the sugarcane genome has expanded relative to the sorghum genome, largely due to the presence of transposable elements and uncharacterized intergenic and intronic sequences. Conclusion This release of sugarcane genomic sequences will advance our understanding of sugarcane genetics and contribute to the development of molecular tools for breeding purposes and gene discovery. (Résumé d'auteur)

Mots-clés : sorghum; métabolisme des glucides; carte génétique; séquence nucléotidique; polyploïdie; génie génétique; génome; saccharum spontaneum; saccharum officinarum; chromosome artificiel

Thématique : Génétique et amélioration des plantes; Physiologie et biochimie végétales

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