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Production of Hevea brasiliensis transgenic lines overexpressing transcription factors involved in ethylene signalling pathway

Lestari R., Rio M., Martin F., Pirrello J., Dessailly F., Leclercq J., Suharsono S., Montoro P.. 2015. In : APRC Asia Pacific Rubber Conference 2015. Phuket : Prince of Songkla University, 1 p.. Asia Pacific Rubber Conference. 2, 2015-10-15/2015-10-16, Phuket (Thaïlande).

The gaseous plant hormone ethylene has a wide variety of applications in agriculture and horticulture. Ethephon, an ethylene releaser, is used to stimulate natural rubber production in Hevea brasiliensis latex cells. Ethylene Response Factors (ERF) are the last transcription factors if the ethylene signalling pathway and control a large number of ethylene-responsive genes. Two Hevea ERF, HbERF-IXc4 ans HbERF-IXc5, are orthologs to ERF1 a jey regulator at the crosstalk of ethylene and jasmonate signalling pathways. These genes were suggested to play an important role in regulating latex cell metabolism in response to tapping and ethephon stimulation. We regenerated transgenic lines overexpressing HbERF-IXc4 ans HbERF-IXc5 under the control of two promoters, 35S CaMV and HEV2.1, respectively. The latter was shown to direct latex-specific expression in non-photosynthetic tissues. The somatic embryogenesis process was affected by these modifications. however, successful plant regeneration has been obtained. Further characterization of this plant material is in progress to understand the function HbERF-IXc4 ans HbERF-IXc5 in latex. (Texte intégral)

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