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Shotgun sequencing of the mitochondrial genome of the Aldabra giant tortoise (Aldabrachelys gigantea)

Besnard G., Thèves C., Mata X., Holota H., Rakotozafy L.M.A., Pedrono M.. 2016. Mitochondrial DNA. Part A, 27 (6) : p. 4543-4544.

DOI: 10.3109/19401736.2015.1101554

The complete mitochondrial genome of the Aldabra giant tortoise [Aldabrachelys gigantea (Schweigger, 1812): Reptilia, Testudines, Testudinidae] was sequenced using a shotgun approach on an Illumina HiSeq 2500 platform (Illumina Inc., San Diego, CA). This genome was 16 467¿bp long and presents the typical organization found in vertebrates. The mean coverage of sequencing was 116×. A phylogenetic analysis of the Testudinidae confirms the placement of Aldabrachelys in an Indian Ocean group (including Madagascar). This mitogenome constitutes a reference for ancient DNA analyses of the extinct Madagascan lineages of Aldabrachelys.

Mots-clés : tortue terrestre; testudinidae; génome; adn mitochondrial; séquence nucléotidique; phylogénie; madagascar; aldabrachelys gigantea; séquencage

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