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Characterization of microsatellites identified by next-generation sequencing in the neotropical tree Handroanthus billbergii (Bignoniaceae)

Morillo Velastegui L.E., Buitron J., Limongi R., Vignes H., Argout X.. 2016. Applications in Plant Sciences, 4 (5) : 4 p..

Premise of the study: We developed microsatellite (simple sequence repeat [SSR]) markers in the Neotropical tree Handroanthus billbergii (Bignoniaceae), to be applied in assessment of genetic diversity in this species as a reference for inferring the impact of dry forest fragmentation in Ecuador. Methods and Results: Using next-generation sequencing, we detected a total of 26,893 putative SSRs reported here. Using an ABI 3500xl sequencer, we identified and characterized a set of polymorphic markers in 23 individuals belonging to three populations of H. billbergii. Conclusions: We report a set of 30 useful SSR markers for H. billbergii and a large list of potential microsatellites for developing new markers for this or related species. (Résumé d'auteur)

Mots-clés : séquence d'adn; polymorphisme génétique; variation génétique; écosystème forestier; forêt tropicale; biodiversité; adn; marqueur génétique; bignoniaceae; tabebuia; Équateur; handroanthus billbergii

Thématique : Génétique et amélioration des plantes; Foresterie - Considérations générales; Ecologie végétale

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