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Current advances in serological diagnosis of Peste des petits ruminants virus

Libeau G.. 2015. In : Munir Muhammad (ed.). Peste des petits ruminants virus. Berlin : Springer, p. 133-154.

DOI: 10.1007/978-3-662-45165-6_8

Peste des petits ruminants (PPR) is a contagious disease with potential for high economic impacts. Convalescent and vaccinated small ruminants develop a strong and lifelong immunity and are fully protected against re-infection. Consequently, the presence of antibodies, whether they are against a wild virus or a vaccine, is a marker of the host immune protection. Thus, specific antibodies cannot be missed with classical and validated diagnosis assays, such as conventional enzyme-linked immunosorbent assay (ELISA), an alternative testing method to the conventional virus neutralization test (VNT), allowing to monitor vaccination or locate disease outbreaks. Recent insights into the virions' protein composition, development of expression systems, recombinant vectors to express protective antigens, have contributed greatly to update our current knowledge about diagnostics. In the light of this progress, the chapter focuses on PPRV serological tests with particular attention to diagnostic antigen targets and refinement of assays for accurate serodiagnosis of PPRV.

Mots-clés : peste des petits ruminants; virus peste petits ruminants; sérologie; immunodiagnostic; technique analytique; protéine virale; protéine recombinante; test elisa; immunofluorescence; hémagglutinine; inhibition; transfert de gène; antigène; anticorps; technique immunologique

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