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Juvenile Hevea transgenic plants over-expressing transcription factor HbERF-IXc5 develop more latex cells than wild-type material

Lestari R., Rio M., Martin F., Leclercq J., Dessailly F., Sanier C., Melliti S., Suharsono S., Montoro P.. 2016. In : CRRI and IRRDB International Rubber Conference 2016, Siem Reap, Cambodia. Siem Reap : CRRI; IRRDB, p. 327-331. International Rubber Conference, 2016-11-21/2016-11-25, Siem Reap (Cambodge).

Mechanical wounding through tapping has long been known to induce laticifer differentiation in Hevea brasiliensis. Recent studies reveal that dehydration, hydrogen peroxide, and jasmonates can similarly play a role in wound-induced laticifer differentiation. The Hevea ethylene response factor gene, HbERF-IXc5, is thought to be an ortholog to ERF1 of Arabidopsis. ERF1 has been suggested as a component of defence responses through the involvement of the ethylene and jasmonic acid signalling pathways. In this study, Agrobacterium tumefaciens-mediated genetic transformation was carried out with this candidate gene. A histological analysis of HbERF-IXc5 lines revealed several changes in anatomy, particularly in terms of the number of laticifer cells. The results suggest that HbERF-IXc5 is a transcription factor driving the expression of some target genes involved in jasmonate-induced laticifer differentiation.

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