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Molecular epidemiology of emerging begomovirus diseases on vegetable crops in Burkina Faso. [O21]

Ouattara A., Tiendrebeogo F., Lefeuvre P., Thébaud G., Chiroleu F., Allibert A., Hoareau M., Traoré E.V., Barro N., Traoré O., Lett J.M.. 2017. In : Livre des résumés des 16 ème Rencontres de virologie végétale. Aussois : CIRAD; CNRS, p. 36-36. Rencontres de virologie végétale, 2017-01-15/2017-01-19, Aussois (France).

Background and objectives begomoviruses (family geminiviridea) are transmitted by Bemisia tabaci and are responsible for serious diseases in the world. African begomoviruses described on crops are mainly monopartite (dna-a component), except cassava-infecting bipartite begomoviruses (dna-a and dna-b components). In recent years, a complex of more than 10 monopartite begomoviruses responsible for tomato (yellow) leaf curl diseases (tylcd-tolcd) has been described on tomato in sub-Saharan Africa [1]. Faced with the upsurge of vegetable virus diseases in Burkina Faso, we have undertaken (1) to identify the causal agents and their molecular diversity, and (2) to investigate their main epidemiological parameters in the field and under controlled conditions. Material and methods forty-five localities from the main vegetable-growing areas of Burkina Faso were surveyed from 2013 to 2016. In total, 2065 leaf samples were collected from cultivated and uncultivated plants. Some of them were subjected to begomovirus diagnosis (sequence-dependent methods) by pcr, rolling circle amplification, cloning and sequencing and others were used for more accurate and complete description of virus communities using a metagenomic approach (sequence-independent methods) based on rca, pcr-random amplification tagging and illumina sequencing. Agroinfectious clones were constructed in order to assess the pathogenicity of some characterized viruses. The transmission rate of these viruses was estimated after acquisition of the virus by B. tabaci. Results we have cloned and sequenced 143 dna sequences (109 dna-a and 34 dna-b). Nucleotide similarity analyses confirmed the presence of at least four known African monopartite begomoviruses (clcugv, pepyvmv, tolmlv and tolcghv). It also revealed for the first time in West Africa the mastrevirus cpcdv on tomato and a new species named “tomato leaf curl Burkina Faso virus”. We characterized a dna-b molecule on cultivated and uncultivated plants, unexpect

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