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Polymorphic microsatellite loci from Dacryodes edulis (Burseraceae), a Central African rainforest and fruit-tree species

Benoit L., Born C., Vignes H., Chevallier M.H., Todou G., Debain C., Joly H.. 2011. American Journal of Botany, 98 (4) : p. e74-e75.

DOI: 10.3732/ajb.1000463

Premise of the study: Microsatellite markers were isolated and characterized from Dacryodes edulis (Burseraceae), a tropical rainforests fruit tree of central Africa. ¿ Methods and Results: Using an enrichment protocol, six microsatellites loci were developed from Dacryodes edulis. We investigated polymorphism using 45 trees from three widely separated populations in Cameroon. All loci were polymorphic, with the number of alleles ranging from 2 to 15. Polymorphism was widely variable among loci and expected heterozygosities ranged from 0.06 to 0.84 with a mean value of 0.49. ¿ Conclusions: These loci will be useful for the in-depth analysis of population structure and phylogeographic variation throughout the distribution range of Dacryodes edulis and other related taxa, Dacryodes buettneri and D. normandii, in which all loci were also amplified. Furthermore, they will offer the opportunity to study early domestication processes acting on the genetic diversity of Dacryodes edulis.

Mots-clés : dacryodes edulis; arbre fruitier; biodiversité; variation génétique; polymorphisme génétique; marqueur génétique; locus; allèle; taxonomie; distribution géographique; domestication des plantes; dynamique des populations; phylogénie; afrique centrale; cameroun

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