Objective: African swine fever, one of the most devastating diseases affecting wild and domestic swine, is due to a large DNA virus, only member of the Asfarviridae family. After ASF introduction in Georgia in 2007, the disease became endemic in the Caucasian region of the Russian Federation and spread towards the Western regions of Europe entering in EU Members States at the beginning of 2014. As no vaccine or antiviral are available to fight against this infection, the only tools to control it are preventive measures based on early detection and actual knowledge of the epidemiological risks. In African sub-Saharan countries, ASF persistence is described to be related to different and complex epidemiological scenarii involving domestic and wild suids and soft tick vectors of the genus Ornithodoros. In EU, one species of Ornithodoros, O. erraticus, is known to be able to maintain and/or transmit some ASFV isolates classified in the genotype I. Recently, the Pirbright Institute also demonstrated that O. erraticus was able to amplify the Georgia2007/1 ASFV (genotype II), at least during 3 months. The objective of the current study was to evaluate the in- vitro and in-vivo transmissibility of the Georgia 2007/1 ASFV by infected O. erraticus ticks. Methods: The Georgia 2007/1 ASFV strain belonging to the genotype II, kindly provided by L. Dixon (OIE reference lab), was grown on porcine pulmonary alveolar macrophages to the titre of 106to 107 HAD50 , then diluted 100-fold into pig blood for tick infection or 1000-fold in medium for intradermal inoculation to pigs. Ticks were captured in Portugal by F. Boinas and mass reared at CIRAD for one year and a half to obtain a stable and mature population. During this period, several techniques of artificial feeding were tested to optimize the method. In December 2014, 60 adults or last nymphal stages -group A- coming both from field and 1st generation laboratory were artificially engorged on pig blood supplemented with Georgia 2