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First report of copper-resistant Xanthomonas citri pv. citri pathotype A causing Asiatic citrus canker in Réunion, France

Richard D., Tribot N., Boyer C., Terville M.A., Boyer K., Javegny S., Roux-Cuvelier M., Pruvost O., Moreau A., Chabirand A., Vernière C.. 2017. Plant Disease, 101 (3) : p. 503.

Xanthomonas citri pv. citri causes Asiatic citrus canker (ACC), which induces erumpent, callus-like lesions on all aerial organs, and consequently defoliation, premature fruit drop, and twig dieback under high inoculum pressure. Present in most tropical and subtropical citrus producing regions, the disease can lead to important decreases in yield and limitations to export markets due to the quarantine status of the pathogen in some countries ( Graham et al. 2004 ). Strains assigned to genetic lineage 1 within pathotype A, which has the widest host range comprising most citrus cultivars and citrus relatives, were shown to be primarily involved in the worldwide expansion of the bacterium over the 20th century ( Pruvost et al. 2014 ). ACC has been considered endemic in Réunion Island since the 1970s, but was likely present earlier and was not subjected to any eradication attempt. Most citrus growers on the island frequently apply copper-based pesticides as part of integrated pest management programs. A recurring lack of ACC control in several citrus groves was investigated in 2014 using our lab collection of authenticated X. citri pv. citri strains. A total of 501 strains from active epidemics as well as 123 historical strains isolated from 1978 onward were tested for copper resistance using PCR assays targeting copL ( Behlau et al. 2013 ). A total of 115 strains produced the expected amplicon whereas others (including all historical strains) and the negative control (H 2 O) did not. Phenotypic tests were carried out on low-complexing mineral salts casitone-yeast extract-glycerol-agar (CYE) plates ( Zevenhuizen et al. 1979 ) flooded with bacterial suspensions ( ? 5 × 10 6 cfu ml ¿1 ) in sterile tris buffer (pH 7.2), allowed to dry for 15 min., inoculated with a sterile CuSO 4 , 5H 2 O solution (10 mg ml ¿1 ) with an easySpiral plater used to create a gradient of log-increasing copper concentrations toward the plate center and incubated for 2 days at 28°C. Using this as

Mots-clés : réunion

Thématique : Maladies des plantes

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