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Presence of 16SrIV phytoplasmas of subgroups A,D and E in planthopper Haplaxius crudus Van Duzee insects in Yucatan, Mexico

Narvaez M., Vázquez-Euán R., Harrison N., Nic-Matos G., Julia J.F., Dzido J.L., Fabre S., Dollet M., Oropeza C.. 2018. 3 Biotech, 8 (1) : 9 p..

The present study was carried out to determine if group 16SrIV phytoplasmas, causing lethal yellowing (LY) disease, are present in Haplaxius crudus Van Duzee (Hemiptera: Cixiidae) insects associated with palms in Yucatán, Mexico. Haplaxius crudus feral insects were captured from palm foliage at two locations (Chicxulub Puerto and CICY, Mérida, where LY-type diseases are active) and evaluated individually for the presence of phytoplasma DNA by a group 16SrIV-specific nested PCR assay. The results showed positive detection in H. crudus insects in a proportion of 2.7% (of the total 2726 analyzed) during a 3-year period of study. The percentage of detection was different for each site, 5.9% positive of 799 insects from Mérida and 1.7% of 1927 from Chicxulub Puerto. Positive detections were also obtained in extracts from 5.3 to 1.2% of males and females, respectively. Sequencing and in silico RFLP and phylogenetic analyses of PCR-amplified rDNA products indicated that H. crudus insects from Chicxulub Puerto harbored phytoplasma strains of subgroups 16SrIV-A or 16SrIV-D, whereas in insects from Mérida the strains found were 16SrIV-A, 16SrIV-D or 16SrIV-E. The diversity of subgroup strains detected in H. crudus coincided with strains previously identified in palms showing LY-type disease syndromes in Yucatán thereby implicating H. crudus as a candidate vector of 16SrIV phytoplasmas in this region of Mexico.

Mots-clés : ravageur des plantes; cocos nucifera; arecaceae; pcr; identification; maladie bactérienne; transmission des maladies; phytoplasme; vecteur de maladie; cixiidae; yucatan; jaunissement mortel; haplaxius crudus

Thématique : Ravageurs des plantes; Maladies des plantes

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