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In-vivo determination of polyisoprene and resins contents of guayule plants by near infrared spectroscopy

Taurines M., Brancheriau L., Palu S., Pioch D., Tardan E., Boutahar N., Sartre P., Meunier F.. 2018. Londres : AAIC, 56 p.. AAIC Annual Meeting. 30, 2018-09-23/2018-09-26, Londres (Royaume-Uni).

Rubber is omnipresent in our daily lives. This elastomer is used to make more than 40,000 products including tires and medical gloves. Because of its exceptional properties, natural rubber is essential for the industry. Furthermore, it is a raw material renewable and environmentally friendly (contrary to synthetic rubber). Today the only commercial source of natural rubber comes from the culture of hevea (Hevea brasiliensis Müll. Arg.). However, the hevea plantations will no longer be sufficient to meet the growing demand of natural rubber in ten years. Guayule (Parthenium argentatum A.Gray) is an alternative source for the production of natural rubber. Global warming favors the development of new crops suited to semi-arid areas and adapted to poor soils as guayule. Current issues of the culture of guayule carry on farming practices, genetic improvement, and the effectiveness of the methods of extraction and synthesis of rubber. This study is carried out under the GuayulSim project, which covers the two first issues. GuayulSim is financially supported by the Labex Agro (ID-1605-026). Direct determinations of polyisoprene and resin contents require a destructive methodology that is very long to be implemented in the laboratory. To make a very large number of measures, it is necessary to determine the contents in a quick way, in a non-destructive way, and directly in the field. The objective of this study is to improve the laboratory method by near infrared spectroscopy developed at Cirad, powerful but requesting time for the biomass collection, drying and grinding, and therefore destructive. The new method will allow making measurements directly on the plant in vivo, saving significant time and without altering the plantation since the picking of plants or branches will be no longer necessary. More specifically, the aim is to: (a) establish a model of determination of polyisoprene and resins in-vivo. (b) To test the reliability of the model. (c) To compare and interpret differences in spectral responses between the field and the laboratory. (d) Finally to determine the optimal number of measures and their locations from the analysis of the variability of contents in the field and on whole plant.

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