Nitrogen (N) is a fundamental nutrient in agroecosystems, but has considerable negative environmental impact when used in excess. Meeting N demand while reducing excess N can be achieved by improvements in N use efficiency (NUE) of a crop agroecosystem. This is generally determined destructively (i.e. by cutting the cane) at the end of the crop cycle during harvest, where NUE is typically higher than during initial phases of crop development. Here we present the findings of a methodological study that aimed at developing non- destructive estimations of NUE throughout the crop cycle. We investigated the ability of allometric relationships to estimate sugarcane biomass by testing different functions and measurable traits (cane height and basal diameter) on six sampling dates. A dilution curve was constructed to predict N content of the sugarcane biomass by harvesting 20 stalks of cane on five separate dates. The number of harvested stalks was then rationalised to reduce the amount of harvested cane without affecting the ability of the curve to predict N content. As N derived from fertiliser (Ndff) can be determined from a representative leaf using an 15N-labelling approach, 15N content of each leaf on three stalks of cane was determined on four sampling dates. This enabled identification of the most representative leaf for determining 15N content of the biomass. The percentage difference and the associated error between sugarcane biomass predicted by allometric models and 2.5m harvested plots varied depending on the combinations of measurable traits used for the allometric relationships. The values for cane: 1) height on each date; 2) height x diameter on each date; and 3) height x diameter using a global relationship across sampling dates was 2±3 %, 2±3 % and 5±3 % respectively. In order to construct an adequate dilution curve, we determined statistically that a minimum of 5 sugarcanes needed to be sampled. The leaf with an 15N content most representative of the enti