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Assessment of the viral disease threats on sorghum and phenotyping support on diseases. [P.29]

Bangratz M., Tibiri E.B., Julian C., Filloux D., Pinel-Galzi A., Brugidou C., Roumagnac P., Tiendrebeogo F., Hebrard E.. 2019. In : Livre des résumés des 17 ème Rencontres de virologie végétale. Aussois : INRA, p. 88-88. Rencontres de Virologie Végétale (RVV 2019). 17, 2019-01-27/2019-01-31, Aussois (France).

Several virus families have been previously reported on sorghum around the world such as Potyviridae (Maize dwarf mosaic virus MDMV, Sugarcane mosaic virus SCMV), Geminiviridae (Maize sætreak virus MSV) or Luteoviridae (Sugarcane yellow leaf virus SCYLV). As their names indicate, these viruses were first described on sugarcane and / or corn before being found on sorghum. Other viruses have been found directly on sorghum such as Sorghum mosaic virus (SrMV, Potyviridae), Sorghum chlorotic spot virus (SCSV, Virgaviridae) or Sorghum stunt mosaic virus (SSMV, Rhabdoviridae). Up to now, the current prevalence of viral diseases in fields is unknown. The difficulties to recognize viral symptoms and the spatiotemporal variability of viral epidemics could explain the lack of data. Now, it appears crucial to assess the epidemic risk and set up an epidemiological surveillance network. An exploratory analysis was conducted at three sites representative of contrasted agroecological conditions of Burkina-Faso. Twenty fields were assessed and fifty leaf samples were collected, they showed mostly Maize streak virus- and Sorghum mosaic virus-like symptoms. Preliminary assay on few samples were performed using nucleic acid amplification with conserved primers of Geminiviridae and Potyviridae families. Partial virus sequencing is ongoing. In parallel, all the samples will be analyzed using an open-mind approach, Virion-associated nucleic acid preparation (VANA) and HiSeq Illumina sequencing. The output of this study will be in the next future the development of inexpensive, fast, standardized and effective diagnostic methods for all currently known or unknown viral diseases on sorghum. In addition to the taxonomic identification, these high throughput sequencing and metagenomics technologies will allow the detection of viral co-infection events. Sonly, the project will include a broader approach including other pathogens such as bacteria and fungus in a view to decipher the synergistic and antagonistic relationships between sorghum pathogens.

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