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A highly specific tool for identification of Xanthomonas vasicola pv. musacearum based on five Xvm-specific coding sequences

Nakato G.V., Wicker E., Coutinho T.A., Mahuku G., Studholme D.J.. 2018. Heliyon, 4 (12) : 11 p..

Xanthomonas vasicola pv. musacearum (Xvm) is a bacterial pathogen responsible for the economically important Xanthomonas wilt disease on banana and enset crops in Sub-Saharan Africa. Given that the symptoms are similar to those of other diseases, molecular diagnosis is essential to unambiguously identify this pathogen and distinguish it from closely related strains not pathogenic on these hosts. Currently, Xvm identification is based on polymerase chain reaction (PCR) with GspDm primers, targeting the gene encoding general secretory protein D. Experimental results and examination of genomic sequences revealed poor specificity of the GspDm PCR. Here, we present and validate five new Xvm-specific primers amplifying only Xvm strains.

Mots-clés : biologie moléculaire; bioinformatique; maladie des plantes; organisme nuisible; agent pathogène

Thématique : Maladies des plantes; Méthodes mathématiques et statistiques

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