Publications des agents du Cirad


An aquaglyceroporin is abundantly expressed early in the development of the suspensor and the embryo proper of loblolly pine

Ciavatta V.T., Morillon R., Pullman G.S., Chrispeels M.J., Cairney J.. 2001. Plant Physiology, 127 : p. 1556-1567.

DOI: 10.1104/pp.010793

In contrast to angiosperms, pines and other gymnosperms form well-developed suspensors in somatic embryogenic cultures. This creates a useful system to study suspensor biology. In a study of gene expression during the early stages of conifer embryogenesis, we identified a transcript, PtNIP1;1, that is abundant in immature loblolly pine (Pinus taeda) zygotic and somatic embryos, but is undetectable in later-stage embryos, megagametophytes, and roots, stems, and needles from 1 year-old seedlings. Analysis ofPtNIP1;1 transcript in embryo proper and suspensor tissues by reverse transcription-polymerase chain reaction suggests preferential expression in the suspensor. Based on comparisons of derived amino acid sequences, PtNIP1;1 belongs to the nodulin-like members of the major intrinsic protein superfamily branch of the aquaporin (major intrinsic protein) superfamily. Through heterologous expression in Xenopus laevis oocytes and the yeast (Saccharomyces cerevisiae)fps1 - mutant, PtNIP1;1 has been shown to be an active aquaglyceroporin.

Mots-clés : pinus taeda; embryogénèse somatique; expression des gènes; transcription inverse; xenopus laevis; saccharomyces cerevisiae; aquaporine

Documents associés

Article (a-revue à facteur d'impact)

Agents Cirad, auteurs de cette publication :