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Feasibility of individual carotenoid quantification in mixtures using UV-Vis spectrophotometry with multivariate curve resolution alternating least squares (MCR-ALS)

Achir N., Servent A., Soto M., Dhuique-Mayer C.. 2022. Journal of Spectroscopy : 13 p..

DOI: 10.1155/2022/4509523

A fast and low-cost analytical method to determine the concentrations of carotenoids (ß-carotene, lutein, and lycopene) from mixed standard solutions or from fruit extracts (kiwi fruits, tomato paste, pink grapefruit juice, kiwi-pineapple smoothie, and apricot nectar) was tested. The methodology was based on UV-Vis spectrophotometry and Multivariate Curve Resolution-Alternating Least Squares (MCR-ALS). Results showed that relative concentrations of ß-carotene, lutein, and lycopene in solvent were successfully determined by this technique with an error inferior to 6%. In real extracts, the procedure succeeded well in identifying the major carotenoid type of the fruit samples but also a more complex profile of a fruit mixture. The results also showed that accuracy of carotenoids determination by UV-Vis spectrophotometry-MCR-ALS in fruit extracts was conditioned by their spectral characteristics (III/II ratios and ¿max), their relative proportion, and the extract purity.

Mots-clés : composition des aliments; analyse quantitative; caroténoïde; extrait de fruit; spectrophotométrie

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