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Agrobacterium mediated transformation of Sri Sumrong 60, a Thai cotton variety

Chaïr H., Kuhapituktum R., Attathom T., Pannetier C., Attathom S.. 1997. Kasetsart Journal. Natural Sciences, 31 (5) : p. 149-156.

Techniques were developed for the induction of embryogenic callus, as well as the transformation and regeneration of Sri Sumrong 60, a Thai cotton variety. Somatic embryogenesis was obtained by culturing hypocotyl explants on MS based medium containing zeatin and NAA and subculturing on an hormone-free medium. This method yielded 23-50% embryogenic callus induction. Transformation of Sri Sumrong 60 with a synthetic crylA(b) gene was achieved by using Agrobacterium tumefaciens strain, with the addition of 100 mM acetosyringone to the medium during inoculation and co-cultivation steps. PCR amplification was used to verify the presence of ctylA(b) in regenerated plants, thus suggesting the successful transformation of the Thai cotton variety Sri Sumrong 60. (Résumé d'auteur)

Mots-clés : gossypium; variété; agrobacterium tumefaciens; embryogénèse somatique; cal; transfert de gène

Article (c-revue sans comité de lecture)

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