Tick and tick-borne diseases (T&TBDs) are a One Health problem affecting both veterinary and public health worldwide. Due to anthropogenic and bio-physical factors (e.g. societal, cultural, economic, political and climatic variables) various tick species' geographic distribution, and thus T&TBD risk, is increasing globally. Vectors of the highest pathogen diversity, ticks are now considered the primary zoonotic disease vector affecting public health in Europe. Today, acaricides are the primary control method, having seemingly profound negative impacts on environmental health, as well as potentially increasing acaricide resistance in tick populations. Therefore, T&TBD risk is simultaneously health-related (human, veterinary and environmental), ecological (ecosystem functions and biodiversity) and economic, depending on local social and ecological parameters that influence this risk. We argue that One Health-inspired tick bite prevention strategies that integrate local societal and biophysical parameters, as well as multi-sector actor collaboration are the most effective T&TBD risk defense. This approach requires local actor T&TBD risk knowledge combined with science-driven data within multi- actor T&TBD risk network(s). For these reasons, we propose a transdisciplinary method that mobilizes participatory approaches involving local actors to create a shared vision of T&TBD risk. Local actor typologies were first identified using participant observations and secondary data (e.g. the literature), followed by semi-structured interviews with 23 local actors to determine needs, priorities and perceptions regarding local T&TBD risk. Finally, participatory workshops were organized with local actors to discuss social-ecological T&TBD risk indicators and potential collective action-oriented tick bite prevention strategies based on a shared vision of local T&TBD risk.