Individual spores of Frankia strain ORS 140102 were allowed to germinate on Qmod medium. Germination percentages were approximately 30%. Colonies from germinated spores were individually picked off and subcultured on two media giving 22 single-spore cultures. Although colony morphology and pigmentation of the single-spore cultures differed, the study of the electrophoretic patterns of enzymes showed that malate dehydrogenase and esterase profiles of the 22 strains and the mother-strain were very similar.