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Molecular and biological characterization of three isolates of geminivirus infecting tomato in Guadeloupe

Urbino C., Gérion A., Caruana M.L.. 1998. In : Mayer R.T. (ed.), Maxwell D.P. (ed.). 2nd international workshop on Bemisia and geminiviral diseases. Program and abstracts = [Deuxième séminaire international sur les maladies à Bemisia et gemini virus. Programme et résumés]. s.l. : s.n., p. 36-36. International Workshop on Bemisia and Geminiviral Diseases. 2, 1998-06-07/1998-06-12, San Juan (Porto Rico).

Since 1989, tomato fields in Guadeloupe have been infested by a geminivirus disease. Symptoms are yellow mosaic, chlorotic mottling, leaf rolling and stunting. A survey showed that the disease was present over all the island. Cloning and sequencing of an isolate lead to the identification of a bipartite geminivirus close to potato yellow mosaic virus (PYMV-G) (Polston et al, 1998). In this study, three other virus isolates were collected from tomato in Grande-Terre and on the leeward coast of Basse-Terre and from whiteflies (Bemisia argentifolii) in Grande-Terre. They were characterized biologically (mechanical and vector transmission, host range) and molecularly. PCR primers based on conserved sequences were used to amplify and sequence the intercistronic region and the N-terminal region of the coat protein gene (CP). Sequence comparisons between these isolates and the clone of PYMV-G were established in order to confirm the occurrence of PYMV in Guadeloupe.

Mots-clés : géminivirus; identification; solanum; guadeloupe; france

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