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Evaluation of resistance to witches' broom by means of bud inoculation

Ducamp M., Thévenin J.M.. 2000. In : Eskes Albertus (ed.), Engels Johannes M.M. (ed.), Lass R.A. (ed.). Working procedures for cocoa germplasm evaluation and selection : Proceedings of the CFC/ICCO/IPGRI Project workshop, 1-6 February 1998, Montpellier, France. Rome : IPGRI, p. 133-134. CFC/ICCO/IPGRI Project Workshop, 1998-02-01/1998-02-06, Montpellier (France).

The bud inoculation test is presented here as an alternative to the belt spray method for comparisons between clones (budded plants or rooted cuttings). During the Workshop a leaf inoculation method, developed by M. Ducamp in Trinidad, was also presented. The leaf method associates the effect of basidiospore germination/ penetration and the phytotoxic effect of glycerol. However, subsequent work carried out with this method showed that it needs further development before it can be recommended in the project. The description of this method is not included in these Proceedings.

Mots-clés : theobroma cacao; résistance aux maladies; infection expérimentale; crinipellis perniciosa; bourgeon; méthode; maladie du balai de sorcière

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