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A solution for the utilization of in vitro culture for mass-diffusion of Coffea arabica elite materials : The direct sowing of somatic embryos produced in a bioreactor

Etienne H., Solano W., Pereira A., Bertrand B., Vasquez N., Barry-Etienne D.. 2000. In : XIX Simposio Latinoamericano de caficultura del 2 al 6 de octubre, San José, Costa Rica. Memoria. Tegucigalpa : IICA-PROMECAFE, p. 273-283. (Ponencias, Resultados y Recomendaciones de Eventos Tecnicos). Simposio Latinoamericano de Caficultura. 19, 2000-10-02/2000-10-06, San José (Costa Rica).

The effect of germination conditions on the morphology of Coffea arabica L. somatic embryos mass-produced in a one-litre temporary immersion bioreactor (RITA7) was studied with emphasis on direct sowing in soil. Using germinated embryos, direct sowing resulted in a highly successful conversion of embryos into plants. Culture density above 1,600 embryos/11-bioreactor positively affected embryo morphology by causing higher embryonic axis elongation (+4-5mm). At this density, addition of a high sucrose concentration (234 mM), 2 weeks before sowing, promoted an increase in effective plant conversion in soil (78%) and a vigorous vegetative growth of the resulting plants. Using this somatic embryogenesis process, 50 000 selected F1 hybrids were recently difunded in America Central.
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