The traditional methods used for studying resistance to A. flavus are not conducive to identifying the specific components or metabolites that directly affect aflatoxin biosynthesis (Flaherty et al., 1995). Moreover, after twenty years of research in this area, no valuable sources of resistance have been found, particularly in the groundnut germplasm. Since conventional breeding methods for controlling aflatoxin are partially effective, novel biotechnology methods are needed to develop pre-harvest host-plant resistance to aflatoxin accumulation (Brown et al., 1999). This research concerns improving knowledge on:.