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Evaluation of the mitochondrial COI gene as a genetic marker for Myndus crudus and other Myndus spp.

Brown J.K., Dollet M.. 2006. In : XLVI Annual Meeting American Phytopthological Society, Cartagena, Colombia, Septembre 12-16, 2006. s.l. : s.n., 1 p.. Annual Meeting American Phytopthological Society. 46, 2006-09-12/2006-09-16, Cartegena (Colombie).

In the Americas and Caribbean, the lethal yellowing (LY) phytoplasma causes a disease of palm transmitted by the leafhopper Myndus crudus (Cixiidae). In Vanuatu and Ghana, foliar decay disease virus affects coconut palm for which M. taffini is a suspected vector. Recently, high mortality in disease-resistant palms has occurred widespread. The availability of a molecular marker would be useful for tracking distribution and dispersal behaviors of Myndus and related vector species in epidemic and non-epidemic areas. We employed PCR and mtCOI primers [F-C1-J-2195, R-L2-N-3014] to amplify a 780bp fragment from insect DNA extracts, and determined the DNA sequence. Phylogenetic analysis revealed three corresponding Myndus clades: adiopodoumeensis (Ghana), crudus (Caribbean), taffinii (Vanuvatu). M. taffini formed two sister-groups. M. crudus formed one group comprising the collection from Oaxaca-MEX, and a second containing FL-USA, Honduras, Jamaica, and Merida-MEX collections. (Texte intégral)
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