Publications des agents du Cirad


Differential strain-specific diagnosis of the heartwater agent: Ehrlichia ruminantium

Vachiery N., Maganga G.D., Lefrançois T., Kandassamy Y., Stachurski F., Adakal H., Ferraz C., Morgat A., Bensaïd A., Coissac E., Boyer F., Demaille J., Viari A., Martinez D., Frutos R.. 2008. Infection, Genetics and Evolution, 8 (4) : p. 459-466. International Congress on Molecular Epidemiology and Evolutionary Genetics of Infectious Diseases. 8, 2006-10-30/2006-11-02, Bangkok (Thaïlande).

DOI: 10.1016/j.meegid.2007.06.001

Ehrlichia ruminantium is the causative agent of heartwater, a major tick-borne disease of livestock in Africa introduced in the Caribbean and threatening to emerge and spread in the American mainland. Complete genome sequencing was done for two isolates of E. ruminantium of differing phenotype, isolates Gardel (Erga) from Guadeloupe Island and Welgevonden (Erwe) originating from South Africa and maintained in Guadeloupe. The type strain of E. ruminantium (Erwo), previously isolated and sequenced in South Africa; is identical to Erwe with respect to target genes. They make the Erwe/Erwo complex. Comparative analysis of the genomes shows the presence of 49 unique CDS and 28 truncated CDS differentiating Erga from Erwe/Erwo. Three regions of accumulated differences (RAD) acting as mutational hot spots were identified in E. ruminantium. Ten CDS, six unique CDS and four truncated CDS corresponding to major genomic changes (deletions or extensive mutations) were considered as targets for differential diagnosis on four isolates of E. ruminantium: Erga, Erwe/Erwo, Senegal and Umpala. Pairs of PCR primers were developed for each target gene. PCR analysis of the target genes generated strain-specific patterns on Erga and Erwe/Erwo as predicted by comparative genomics, but also for isolates Senegal and Umpala. The target genes identified by bacterial comparative genomics are shown to be highly efficient for strain-specific PCR diagnosis of E. ruminantium and further vaccine management tools.

Mots-clés : génome; ehrlichia; diagnostic; maladie bactérienne; génie génétique; séquence nucléotidique; identification; provenance; ehrlichia ruminantium; afrique; afrique du sud; guadeloupe; france; séquencage

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