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Genetic conformity assessment of C35 citrange seedlings by SSR markers : [P6]

Constantino G., Casabianca S., Poullet T., Appere M., Paolacci V., Luro F., Ollitrault P., Bouffin J.. 2008. In : XIth International Congress Citrus, book of abstracts, Wuhan, China, 26-30 october 2008. s.l. : s.n., p. 121-121. International Citrus Congress. 11, 2008-10-26/2008-10-30, Wuhan (Chine).

C35 citrange rootstock has been widely investigated in the last decades in .mast countries that produce Citrus and knows an increasing interest of the citrus growers. Now day, this rootstock is available for clementine growers in Corsica owing to the good yield and good fruit quality conferred to the clementine. In order to use this rootstock for agronomic trials, we investigated its genetic conformity. For this purpose, we selected C35 seed1ings by discarding off-type seedlings by visual evaluation. We then characterized the molecular conformity of those rootstocks: DNA of eighty six selected plants was extracted from leaves. Five SSR markers presenting heterozygous profiles for C35 were used in order to discriminate zygotic plants. Twenty eight percent of the plants were proved to be zygotic. So it can be expected that a higher percentage of the initial plants was zygotic. ln order to confirm this result, we currently investigate a new set of C35 seedlings not submitted to visual selection as well as a set of Carrîzo citrange seedlings. Indeed, Carrizo has been largely investigated and is known to have a low percentage of zygotic plants. If C35 rootstock presents such a large percentage of zygotic in its seedlings, it might induce risks of yield or fruit quality heterogeneity in commercial orchards as well as the lost, by genetic segregation, of resistance for very important diseases such as Tristeza or Phytophthora. (Texte intégral)

Mots-clés : citrange

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