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Evaluation of a commercial competitive ELISA for the detection of antibodies to Rift Valley fever virus in sera of domestic ruminants in France

Cetre-Sossah C., Billecocq A., Lancelot R., Defernez C., Favre J., Bouloy M., Martinez D., Albina E.. 2009. Preventive Veterinary Medicine, 90 (1-2) : p. 146-149.

This paper describes the sensitivity and specificity of a commercial competitive ELISA for the detection of antibodies to Rift Valley fever virus (RVFV) from sera of French domestic ruminants. Field samples were collected from mainland France for the known-negative sera (cattle = 191, goats = 119, sheep = 192) and from ruminants of a French overseas territory (Mayotte) for the known-positive sera. A cut-off value of 43% was determined for all species, achieving a sensitivity and specificity of 100% and a concordance of 100% with the species-specific threshold recommended by the manufacturer. Our results demonstrate that this ELISA may be a suitable diagnostic tool for disease surveillance programmes and import/export veterinary certification of French cattle, goats and sheep. (Résumé d'auteur)

Mots-clés : test elisa; virus de la fièvre de la vallée du rift; ruminant; france; fièvre de la vallée du rift

Thématique : Maladies des animaux

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