A field method has been developed to quantify root length density (RLD) from root intersection density (RID) measured on a trench-profile, using modelling RID-RLD relationships. For 2D spatial distribution mapping of RID (at 5-cm scale for example), the large amount of data is processed and converted into RLD and root distances (ARD) through modeling. Calculations and RLD mapping can be performed quickly using a new freeware: RACINE2, tailored to this field method. The software also allows a simple modeling of potential root exploration ratio in the soil (PRER) taking ARD into account. The software contains published models calculating RLD from RID for several crops (maize, sorghum, sugarcane, rice), ARD from RLD and PRER from RD. Models may be changed or added into RACINE2. RLD, ARD and PRER are calculated for each spatial unit. They can be mapped. Data can be exported to a spreadsheet or a surface mapping software for further analysis. It is also possible to import data into RACINE2 from a spreadsheet. RACINE2 thus makes studies about root-soil interactions, root growth and root uptake easier. Some examples of field results calculated by RACINE2 are presented (RLD, ARD and RER profiles and maps). They point out differences of PRER when taking (or not taking) into account 2D spatial root distribution. Taking into account spatial variability of root system in relation with soil characteristics may be important for root water and nutrient uptake in field conditions.