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Identification of Mycoplasma mycoides subsp. mycoides small colony genes coding for T-cell antigens

Totté P., Mather A., Reslan L., Boublik Y., Niang M., Du Plessis D.H., Dedieu L.. 2010. Clinical and Vaccine Immunology, 17 (8) : p. 1211-1216.

Genes of the Mycoplasma mycoides subsp. mycoides small colony biotype (MmmSC) coding for proteins capable of eliciting protective T-cell memory responses have potential for incorporation into a recombinant subunit vaccine against contagious bovine pleuropneumonia (CBPP). Here we used lymphocytes from cattle that had completely recovered from infection to screen products of MmmSC genes for recognition by CD4+ effector memory (Tem) and central memory (Tcm) T lymphocytes. Six MmmSC genes (abc, gapN, glpO, lppA, lppB, and ptsG) were expressed as histidine-tagged recombinant polypeptides, or synthetic overlapping peptides, before inclusion in proliferation and gamma interferon (IFN- ) assays. Only two MmmSC antigens, LppA and PtsG, consistently induced recall proliferation from immune CD4+ T cells and IFN- production in all animals tested. Moreover, LppA and PtsG were shown to possess epitopes recognized by both short-lived CD4+ Tem and long-lived CD4+ Tcm cells. (Résumé d'auteur)

Mots-clés : gène; immunostimulant; immunologie; protéine; antigène; vaccin; biotype; bovin; identification; mycoplasma mycoides; péripneumonie contagieuse bovine; afrique au sud du sahara

Thématique : Maladies des animaux; Génétique et amélioration des animaux

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