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Determination of the geographical origin of fruits by using 26S rDNA fingerprinting of yeast communities by PCR-DGGE: An application to shea tree fruits

El Sheikha A.. 2010. International Journal of Life Sciences, 4 (6) : p. 9-15.

International trade intensifies the process of globalization which has extended to the entire planet and extends to the entire planet. In view of the difficulties of installing these documentary systems in developing countries, in particularly the countries of sub-Saharan Africa, the new strategies of traceability emerge. Among the new tools of tracing the products of vegetable origin, a "biological code bar" based on the analysis of the DNA of micro-organisms present on the fruits is an interesting tool. A molecular technique employing 26S rDNA profiles generated by PCR-DGGE was used to detect the variation in yeast community structures of Shea tree fruits (Vitellaria paradoxa) from Senegal, Ghana and Mali. When the 26S rDNA profiles were analyzed by image analysis and multivariate analysis, distinct microbial communities were detected on Shea tree fruits. The band profiles of yeasts from different countries were specific for each location and could be used as a bar code to discriminate the origin of the fruits. This method is a new traceability tool which provides fruit products with an unique biological bar code and makes it possible to trace back the fruits to their original location. (Résumé d'auteur)

Mots-clés : fruits; provenance; identification; levure; Électrophorèse sur gel; empreinte adn; pcr; Étude de cas; vitellaria paradoxa; micro-organisme; technique analytique; sénégal; ghana; mali

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