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Nucellus friable callus induction and its embryoid ontogenyfrom Rubber tree clone Reyan88-13 Hevea brasiliensis Müll. Arg.)

Zhe L., Xuemei D., Aihua S., Tiandai H., Quannan Z., Huasun H., Weifu L., Lardet L., Montoro P., Carron M.P.. 2010. Journal of Tropical Organisms, 1 (4) : p. 308-314.

The nucellar tissue from the young seeds of rubber tree clone Reyan88-13 was used to induce embryogenic callus. The different culture parameters such as the different types and concentrations of plant growth regulators and different calcium concentrations had been studied in embryogenic caHus induction media and subculture media. The proper culture parameters had been determined. Via continuous subculture and selection during 5 months, friable and embryogenic callus had been gradually obtained. Long-term subculture had been carried on for the friable and embryogenic calli. Histological sections proved that the calli proliferated by long-term subculture maintained the embryogenic characters. The nucellar friable and embryogenic calli of rubber tree clone Reyan88-13 that had been subcultured for more than 2 years were used for inducing embryoids. At present 186 embryoids were obtained. These embryoids were being used to induce plant regeneration. (Résumé d'auteur)

Mots-clés : régénération in vitro; clone; culture d'embryon; milieu de culture; embryogénèse somatique; développement biologique; hevea brasiliensis; chine

Thématique : Multiplication végétative des plantes; Physiologie végétale : croissance et développement

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