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Combined sanger and ngs sequence analysis of the myostatin gene (mstn) in the Camelus dromedarius species

Muzzachi S., Burger P., Fitak R.R., Oulmouden A., Cherifi Y., Yahyaoui H., Zayed M.A., Lacalandra G.M., Faye B., Ciani E.. 2015. Veterinariâ, 42 (2) : p. 353-355. 4th Conference of the International Society of Camelid Research and Development/ISOCARD 2015. 4, 2015-06-08/2015-06-12, Almaty (Kazakhstan).

Different mutations have been identified in the myostatin gene (MSTN), some of which are responsible for protein inactivation and double muscling phenotype in mammals. So far, no extensive polymorphism survey has been carried out in Camelus dromedarius. We therefore performed a sequence analysis, adopting a combined strategy involving Sanger and next generation sequencing (NGS). Notably, 3.6 kb of the MSTN locus were Sanger sequenced in a population dataset including samples from Algeria (10), Tunisia (5), Egypt (9), Mauritania (5), Sudan (5) and Saudi Arabia (9). A further wholegenome dataset, including 7 C. dromedarius from Pakistan (1), Kenya (1), Saudi Arabia (3), Canary Islands (1) and Oman (1) were sequenced using the Illumina Hi-Seq 2000 technique at an average 15-fold coverage. Whole-genome NGS sequence data from 9 C. bactrianus and 7 C. ferus samples were also available for comparison. Overall, only four polymorphisms were detected, all of them were observed in intronic regions, corresponding to an average presence of one SNP per 1200 bps. Ten fixed sites were observed when comparing C. dromedarius MSTN sequences with those from C. bactrianus and C. ferus. The apparent low sequence diversity observed at the MSTN locus may reflect the peculiar evolutionary history of this species, with purifying selection and drift phenomena as the most likely acting forces.

Mots-clés : dromadaire; facteur de croissance; séquence nucléotidique; mutation; polymorphisme génétique; algérie; tunisie; Égypte; mauritanie; soudan; arabie saoudite; pakistan; oman; canaries (îles); kenya; camelus dromedarius; séquencage

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