Highly Pathogenic Avian Influenza H5N1 (HPAI) virus is now considered endemic in several Asian countries as well as in Egypt. In Cambodia, the virus has been circulating in poultry population since 2004, with a dramatic effect on farmers' livelihoods. HPAI H5N1 is also a public health threat as 56 human cases (including 39 deaths) have been identified so far in this country. In Thailand, no outbreaks have been recently reported, but surveillance and control are still important to prevent any new HPAI H5N1 incursion. Risk maps may efficiently inform disease surveillance and control systems. However, in countries with a lack of primary care systems, their production can be hampered by lack of reliable disease data. In such situations, knowledge-driven modeling methods including spatial multicriteria decision analysis (MCDA) have been identified as an alternative to classical statistical approaches. However, these methods can appear somehow subjective and poorly connected to real data. Validation of knowledge-driven maps is therefore an essential and challenging task. The present study used MCDA to map HPAI H5N1 risk in domestic poultry in Southeast Asia. In a first step, 3 MCDA models (2 with weights calculated from literature review, and 1 with weights attributed by experts) were built and applied to Thailand, where reliable HPAI H5N1 data exist. In a second step, the best model was applied to Cambodia where the number of poultry cases is under-estimated due to poor reporting by farmers. For each country, the same set of 10 predictors was selected. Predictors included density of farm and free-grazing ducks, backyard chicken density, proportion of rice fields and number of rice crops in a 2- and 5-km radius, wetlands density, altitude, human population density and proximity to major cities. 14 and 11 experts were contacted in Thailand and Cambodia, respectively. They were asked i) to choose a relationship between each selected variable and the risk of HPAI H5N1 and ii