The coexistence of protected areas and neighbouring communal areas in Southern Africa is jeopardised by negative interactions between wildlife and villagers and their livestock. The African buffalo (Syncerus caffer) has often been blamed for a significant proportion of the “human-wildlife conflicts”, mainly through the transmission of pathogens to cattle, competition with livestock for grazing and water resources, and also occasionally involved in crop destruction and threat to people's physical integrity. We present the results of several multidisciplinary studies carried out since 2008 on buffalo-cattle interactions at the periphery of Gonarezhou National Park, Hwange National Park and adjacent communal lands in Zimbabwe. Movements of sympatric buffalo and cattle have been described at various scales using GPS collars, revealing daily/seasonal patterns and occasional events of long-distance dispersal of young buffalo females. Habitat preferences, especially for open grassland habitats, were similar between the two species, and surface availability was the key determinant of their distributions. Overall, buffalo appeared to avoid cattle, although their ranges overlapped more extensively with cattle in Gonarezhou area than in Hwange. We defined contacts between buffalo and cattle using spatial-temporal windows compatible with the transmission of parasites and pathogens through direct contact or indirect contact with contaminated environment. Results of serological tests for various pathogens and analysis of their relationship with buffalo/cattle contacts rates confirmed the likely role of reservoir played by buffalo for several diseases (FMD, theileriosis…), whereas the results were inconclusive for several others (brucellosis, tick-borne diseases, BTB…). Socio-economic surveys carried out in the study areas highlighted the importance of livestock keeping for small-scale farmers' livelihoods. Livestock management strategies have been elicited using participatory app