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Report on the visit to the Marihat in vitro culture laboratory, from 13th to 22nd March 1991

Durand-Gasselin T.. 1991. Paris : CIRAD-IRHO, 20 p.. numero_rapport: 2361bis.

The visit to the PP Marihat in vitro culture laboratory from 18th to 22nd March 1991 showed that progress had been made on the programme. The following recommendations were made, so as to optimize the procedure and the installations. The laboratory's cloning capacity should be used to the full, due to the large number of ortets selected and the need to renew the germplasm constantly. It is therefore best to take 1 to 2 samples per week, to reach a cloning rate of 50 individuals per year. Callus formation is slow but it is possible to sufficient material to implement the procedure embryogenesis rate on calli is still remarkable. Insofar as production quality depends on embryoid clump selection from established cultures, it was recommended that the cultures be sorted very strictly so as to keep only the material that satisfies the characteristics laid down. It was pointed out once again that the main aim of ramet production is to test each clone before distribution, insofar as certain clones may not in fact lead to the expected improvements. This opportunity was taken to recall all the recommendations made on clonal material production and delivery. From a technical point of view, the improvements made over the past few years were recommended, for the caulogenesis stage (use of bottles and a liquid medium) and rhizogenesis (induction and expression in a liquid medium). The results obtained during the last stages outside the laboratory could be improved, by taking greater precautions with watering, which is often too frequent, during the weaning stage, and by using a lighter substrate in the prenursery. Lastly, it would be advisable for the plantings set up to be systematically observed at least once after they begin flowering, so as to determine the fidelity of the material produced by the PP Marihat unit.

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