As ammonia addition is the most widespread latex stabilization method, we investigated the impact of ammonia-stabilization on biochemical and physicochemical indicators of latex in liquid and dry state (thin film) at different storage times (0 to 41 days). For liquid state, measured indicators included surface tension, pH, dry rubber content, total solid content, lipid and protein contents. In addition, semi-quantitative data on lipids and proteins were obtained by TLC and SDS-PAGE, respectively. For latex at dry state, thin films were investigated by AFM to unravel their morphology as well as their elastic and adhesive properties. Analyses were completed by infrared spectroscopy and wettability measurements on non-washed and washed latex films to investigate the impact of film washing as well. The ammonia-stabilization method was shown to quickly and significantly impact the lipid and protein contents resulting in drastic changes regarding the morphology and properties of films.