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Diagnostics through Ralstonia solanacearum genomics: grasping the broad diversity of a complex plant pathogen. [S4-O4]

Cellier G., Ailloud F., Hostachy B., Prior P., Robène-Soustrade I.. 2014. In : The 13th International Conference on Plant Pathogenic Bacteria. Programme and abstracts. Shanghai : SCPBD, p. 93-93. 13th International Conference on Plant Pathogenic Bacteria, 2014-06-08/2014-06-13, Shanghai (Chine).

designed to identify 17 phylogenetic clusters of diagnostic interest, including species, phylotype or sequevar-specific probes along with control probe . Highlight was given on three ecotypes in the phylotype II: (i) Brown rot trains from sequevars IIB-1 ; (ii) Moko disease-causing srains from sequevars IIB-3 IIB-4, IIA-6 and IIA-24, that cause wilt on banana plantain, and Heliconia; and (iii) new pathogenic variants from sequevar IIB-4NPB that lacks pathogenicity to banana Cavendish but can infect many other plant species, including Cucurbitacae. As a first step, a spotted array glass slide format was chosen for its probe screening capacity, where the 100 best 50mers probes out of more than l 000 were selected on 80 strains for switching to a more convenient microarray format, based on the "Array Tube" technology from Alere Technologies, which allows micro-reactions within a vial. Several advantages to witch to this technology will be presented along with first results.

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