This research focused on the "Energy valorization of lignocellulosic products and their by-products for the protection of bio-materials", is dedicated to the valorization of the sugar cane bagasse from Reunion Island (Oversea territory, France), a region where the sugar industry plays an important role in the agricultural sector. The objectives of this research were to: (i) characterize the biochar and the bio-oil according to the pyrolysis process parameter and the biomass used, and to (ii) evaluate the potential of said bio-oil as wood preservatives. Pyrolysis of bagasse was conducted using an experimental set up of fixed bed reactor. Various temperatures (T = 400 °C, 500 °C) and holding time th (30 and 60 minutes), and a heating rate of 10°C/minutes were applied. Pyrolysis products, consisting of biochar and bio-oil, were recovered for further analysis. The biochars were characterized for their proximate and ultimate analysis for energy valorization. For evaluating the anti fungal efficacy of the recovered bio-oil, we conducted fungal growth inhibition tests against two types of wood-decaying fungi: Coriolus versicolor (white-rot) and Coniophora puteana (brown-rot) on Petri dishes, as well as decay tests on treated wood samples.