Introduction - The Guayule (Parthenium argentatum, Asteraceae), is a branched shrub native to northern Mexico showing a growing interest in research and agriculture (Ray, 1993). However, few studies have been done on the structure or functioning of the species and the production itineraries in relation to natural rubber production are still to assess. This study aims to propose a first FSPM of the species using the GreenLab model, calibrated with data issued from two varieties in different environmental conditions. The objective is to understand the interaction between the development of Guayule and natural rubber production. Materials and Methods - Experimental plots were conducted on two varieties (Fig. A), named CL1 (parent USDA 11591 triploid) and CLA1 (parent USDA AZ 101 tetraploid). The CL1 variety has small stem and leaf size compared to the CLA1 variety. These varieties were planted in the field under various environmental conditions related to density (9091 and 62500 plants/ha), and hydric pressure (100%, 66% and 33% of optimal supply). The measurements carried out to count number of phytomers per module as well as the number of relay axes. We also clustered the total dry weight of leaves and stems per module in order to build the axis organic series (Buis and Barthou, 1984) and to estimate the content of natural rubber from a NIRS analysis (Taurines et al., 2019). The field measurements from the series constituted a target to calibrate the GreenLab model functional parameters such as the organ sink functions (Kang et al, 2018).We chose such a cohort model, since the structure (here the module rank) can be analysed in the biomass production without detailed explicit geometrical computations. Results and Discussion - Experimental results. The Guayule shows a sympodial development composed of modules with a terminal inflorescence. Its architecture corresponds to the Leeuwenberg's model (F. Hallé et al. 1978) producing eight to nine modules per year. The numbe