Transitioning toward minimum or no tillage is challenging for smallholder farmers in sub-Saharan Africa (SSA), due to the possible yield penalties during the initial years of a transition. Understanding the early impacts of such transitions is crucial in a cash crop such as cotton, on which farmers rely for their income, and is necessary to inform agroecological strategies to cope with both these challenges. This study explores the combined impact of minimum or no tillage and fertilizer regimes on agronomic parameters of cotton–cereal rotations, as practiced by smallholder farmers in Benin. A multilocation experiment was set up in three different agroclimatic zones, namely, Savalou (7°55'41¿, 1°58'32¿), Okpara (2°48'15¿, 7°72'07¿), and Soaodou (10°28'33¿, 1°98'33¿). In each area, the experiment was laid out as a split-plot design with four replications (main plot = soil preparation; subplot = fertilizers regimes). The treatments consisted of three different forms of soil preparation, namely, tillage, strip tillage, and no tillage or direct seeding, and four fertilization regimes, namely, basal mineral fertilizers (BMF, 200 kg ha1 of N14P18K18S6B1 + 50 kg ha1 of urea), BMF + A (200 kg ha1 of calcium phosphate amendment, 22P2O5-43CaO-4S), BMF + C (400 kg ha1 of compost), and BMF + A + C. At all sites, direct seeding led to lower below-ground biomass growth and seed cotton yields compared with conventional tillage in an early transition to conservation agriculture starting from degraded soils (2% to 25%). Weak rooting under direct seeding resulted in lower cotton yields compared with that under tillage (-12%) and strip tillage (-15%). At 45 and 90 days after emergence, cotton plants were shorter under direct seeding compared with tillage (-9% and -13%, respectively) and strip tillage (-23% and -6%, respectively). Fertilizer regimes affected seed cotton yields differently across sites and treatments, with marginal responses within soil preparation methods, but they cont