A variety of imaging sensors are currently used in research and commercial practices to quantify complex crop traits for breeders. Imaging technology is a fast, non-destructive high-throughput phenotyping tool that has been used widely for accurate acquisition of crop traits on a large scale. The DigiEye is a computer-controlled digital camera system for measuring colour and capturing high-quality repeatable images. The SOP highlights the procedure of calibrating the DigiEye, for accuracy and consistance of results.