The article reports the AMS (accelerator mass spectrometry) radiocarbon investigation of the historic Grand Baobab of Mahajanga. The largest African baobab of Madagascar exhibits a cluster structure, which consists of 6 fused ordinary stems and of 3 small binding stems. Two samples were collected from the largest stem and from a primary branch, out of which several tiny segments were extracted and dated by radiocarbon. The oldest dated sample segment had a radiocarbon date of 214 ± 17 BP, which corresponds to a calibrated age of 265 ± 25 calendar years. The dating results indicate that the Grand Baobab of Mahajanga is 275 ± 25 years old.