To prepare quantities of reference materials for stickiness measuring methods (RefMat), the main goal is to assure that the sticky points are evenly distributed within the material. This is achieved only when the statistical distribution of the sticky points as detected by Sticky Cotton Thermo-detector (SCT) or High-Speed Stickiness Detector (H2SD) on several sub-samples is a Poisson distribution 1. To approach this condition with cotton fiber samples, opening and doubling the material several times is the ultimate solution, based on over 30 years of experience. Along time, orders have been coming by sets of 100 grams each, and therefore the preparation procedure has been designed accordingly. The following slides illustrate the way RefMat are produced, using a minicard equipped with flat flats in place of teeth flats. Other preparation device could be used as long as thin layers are pilled-up into fleeces, so that the number of doublings allows the possibility to get a Poisson distribution of the sticky points into the RefMat. This procedure takes into account the fact that the minicard breaker removes waste and some fibers along the way. It also takes into account the fact that each set of RefMat is re-established at the time of preparation, as stickiness can change over time. In this way, any user can be sure of the number of sticky points in the RefMat at the moment of delivery; this assumes that RefMats are stored in dry conditions, prior to use after a period of conditioning in the standard atmosphere approved for testing.