Dry weight is used to estimate growth for modelling purposes. Dry weight is commonly derived from fresh weight, which is easily measurable, and dry matter content (DMC). DMC depends on plant physiology and may be variable among organs. DMC distribution within a tree and its sources of variability are poorly investigated, particularly in mango. The objectives of this work were (i) to determine the distribution of DMC among the organs of an irrigated mango tree, and (ii) to assess to which extent the individual plant, the drying method (freeze-drying or oven-drying), the phenological stage and irrigation affect DMC. A distribution of DMC among 22 organs of irrigated whole mango tree has been presented, from fine roots to inflorescences. The drying method did not affect DMC measurement, but the sampling procedure did: radial core samples in woody organs over-estimated DMC. Taproot had the highest DMC (53.4%) which did not differ significantly from DMC of the main woody structure, from stump to scaffolds. Individual plant did not affect DMC of organs, on the contrary to phenological stage. Irrigation affected DMC of taproot, fine and medium roots at the end of harvest, probably in relation to carbohydrate mobilization and soil water status. Our results are used to formulate practical recommendations for sampling procedures, and suggest further studies on carbohydrate cycle in mango.